Standardise membrane properties

Alterations in neural membrane phospholipid components cannot only influence crucial intracellular and intercellular signaling but also alter many membrane physical properties such as fluidity, phase transition temperature, bilayer thickness, and lateral domains. A deficiency of docosahexaenoic acid markedly affects neurotransmission, membrane-bound enzyme and ion channel activities, gene expression, intensity of inflammation, and immunity and synaptic plasticity.

Horrocks L.A. and Farooqui A.A., Prostaglandins Leukotrienes and Essential Fatty Acids, 2004.

Approfondimento

Membrane properties, such as fluidity, permeability, and anchoring of membrane proteins, considerably depend on their membrane phospholipid fatty acid composition.
The cell membrane, prime and fundamental actor of every cellular process, has not been considered as a variability-introducing factor, despite the fact that changes in lipid composition alter its functional characteristics and biophysical properties, therefore influencing biologic processes and introducing negative effects on experimental consistency.
In the cell culture technology, culturing conditions and animal serum variability modify this composition through the life of the culture and cause fluctuation of membrane properties, consequently influencing bio-processes and experimental data and marking the membrane as a primary source of cell culture variability.
With the use of Refeed and Report, Remembrane aims at standardising cell culture membranes in time, thus achieving constant biophysical properties throughout the life of the culture, which would guarantee a better accuracy between experiments carried out in the same labs or in different ones.

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