Refeed® - a personalized solution to improve cell culture experimental models and reduce in-vivo/in-vitro dichotomy.

Relying on its proprietary technology, Remembrane develops Refeed® lipid supplements to be added to existing culture media, reinstating or recreating in vitro the membrane lipid content of the corresponding in-vivo cells.


Due to significant alterations of the lipid content of cell membrane network and of its biophysical properties, in-vitro cells differ greatly from the correspondent in-vivo cells, thus introducing substantial biases in most in-vitro studies (Lamaziere et al., 2013). Clearly, such biases lead companies and laboratories to a considerable waste of time and important loss of money.

Tumoral Cell Lines

Human Lymphocytes
Fatty acid profile of human T lymphocytes extracted from the whole blood of healthy individuals, consistent with data already available in the literature.

Jurkat cells
Fatty acid profile of the cell membrane network of Jurkat cells grown according to the standard protocol currently used in bio-laboratories.

Primary Cells

Mouse Embryonic Fibroblasts – Passage 0
Fatty acid profile of the cell membrane network of Mouse Embryonic Fibroblasts extracted from 12-day-old mouse embryos (vaginal plug, day 0).

Mouse Embryonic Fibroblasts – Passage 3
Fatty acid profile of the cell membrane network of Mouse Embryonic Fibroblasts extracted from 12-day-old mouse embryos (vaginal plug, day 0) and grown in-vitro according to the standard protocol currently used in bio-laboratories.

Stem Cells

Adipose Stromal Cells – Passage 0
Fatty acid profile of the cell membrane network of Adipose Stromal Cells extracted from adipose tissue by enzymatic digestion.

Adipose Stromal Cells – Passage 3
Fatty acid profile of the cell membrane network of Adipose Stromal Cells extracted from adipose tissue by enzymatic digestion and grown in-vitro according to the standard protocol currently used in bio-laboratories.

Refeed® is a lipid supplement for cultured cells reinstating the same membrane network content of the in-vivo cells, including all physiological or pathological conditions.

Remembrane proposes its proprietary Refeed® technology for cell culture users to obtain:

  • Restored in-vivo membrane biophysical properties for cultured cells;
  • Different membrane network phenotypes of typical conditions and pathologies;
  • Accurate in-vitro membrane physiology for cell culture models;
  • More reliable data based on an increased performance of experimental models.

Refeed® is a completely defined combination of lipids and antioxidants. Refeed® is intended to be added as a supplement to standard bovine serum supplemented media, Serum-Free media and Chemically Defined media. Choose the most suitable Refeed® for your research and improve your experimental models.

Personalization
The lipid profile varies considerably even among cells originating from the same tissue, because each cell line or cell type has its own metabolism, pathways of synthesis and specific regulation modality of membrane properties.

Therefore, Refeed® supplements are specifically calibrated for each cell line or cell type. Ask Remembrane for additional ad-hoc Refeed® supplements tailored to your specific membrane remodeling needs.

The dichotomy between in-vivo and in-vitro fatty acid profile varies greatly. This is why Remembrane proposes its Refeed® solution. Here below three examples for Jurkat cells:

Wild-Type
Developing Jurkat cells featuring a membrane network consistent with the in-vivo membrane network of healthy individuals, thus recreating a membrane network characterized by the same physiological properties. Composition: Saturated Fatty Acids, Omega-6 Fatty Acids, Omega-3 Fatty Acids, Cholesterol, Antioxidants.

Cardiovascular Disease-like
Developing Jurkat cells featuring a membrane network reflecting the one of lymphocytes of individuals following a typical western diet, which is a risk factor for Cardiovascular Diseases, with a 12:1 ratio Omega-6/Omega-3 (Lauritzen et al., 2001; Gerrior et al., 2004) and consistent levels of trans fatty acids (Allison et al., 1999) as hallmarks. Composition: Saturated Fatty Acids, Omega-6 Fatty Acids, Trans Fatty Acids, Cholesterol, Antioxidants.

Omega-3 Plus
Developing Jurkat cells featuring a membrane network reflecting the one of lymphocytes of individuals following a typical Omega-3 enriched diet, with a 1:1 ratio Omega-6/Omega-3 and low levels of trans fatty acids and cholesterol as hallmarks (Kuipers et al., 2011; Lassek & Gaulin, 2011). Composition: Saturated Fatty Acids, Omega-6 Fatty Acids, Omega-3 Fatty Acids, Cholesterol, Antioxidants.

Refeed® supplements featuring a membrane network reflecting the one of lymphocytes of individuals affected by specific types of Leukemias or Lymphomas, or of lymphocytes with chronically-inflammated or oxidative-stressed membranes are also available.

Getting a personalized Refeed® is easy: contact us by hitting on the GET A QUOTE button, tell us more about your specific projects and needs, wait for your personalized Refeed® to be ready and shipped to you.

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